Department of Microbiology, Osmania University, Hyderabad-500 007, India.
World J Microbiol Biotechnol. 1996 Jan;12(1):57-60. doi: 10.1007/BF00327802.
Two Clostridium thermocellum strains were improved for ethanol tolerance, to 5% (v/v), by gradual adaptation and mutation. The best mutant gave an ethanol yield of 0.37 g/g substrate, with a growth yield 1.5 times more than its parent. Accumulation of acids and reducing sugars by the mutant strain with 5% (v/v) ethanol was lower than that of the parent strain with 1.5% (v/v) ethanol.
两种嗜热梭菌菌株通过逐步适应和突变得到了乙醇耐受性的提高,达到 5%(v/v)。最佳突变株的乙醇产率为 0.37 g/g 基质,比其亲本的生长产率高 1.5 倍。在 5%(v/v)乙醇下,突变株的酸和还原糖积累低于在 1.5%(v/v)乙醇下的亲本菌株。