Institut für Mikrobiologie der Universität Göttingen, Grisebachstrasse 8, D-3400 Göttingen, Federal Republic of Germany.
Appl Environ Microbiol. 1989 Jul;55(7):1829-34. doi: 10.1128/aem.55.7.1829-1834.1989.
An organism identified as Pseudomonas putida was isolated from an enrichment culture with 2-furoic acid as its sole source of carbon and energy. The organism contained a 2-furoyl-coenzyme A (CoA) synthetase to form 2-furoyl-CoA and a 2-furoyl-CoA dehydrogenase to form 5-hydroxy-2-furoyl-CoA as the first two enzymes involved in the degradation. Tungstate, the specific antagonist of molybdate, decreased growth rate and consumption of 2-furoic acid but had no influence on growth with succinate. Correspondingly, the 2-furoyl-CoA dehydrogenase activity decreased when the organism was grown on 2-furoic acid in the presence of increasing amounts of tungstate. The addition of molybdate reversed the negative effect on 2-furoyl-CoA dehydrogenase activity, which points to the involvement of a molybdoenzyme in this reaction. Both enzymes studied were inducible. No plasmid was detected in this organism.
从以 2-糠酸为唯一碳源和能源的富集培养液中分离到一株被鉴定为恶臭假单胞菌的微生物。该微生物含有 2-糠酰辅酶 A（CoA）合成酶以形成 2-糠酰-CoA 和 2-糠酰-CoA 脱氢酶以形成 5-羟基-2-糠酰-CoA，这两种酶是降解途径中的前两个酶。钨酸盐是钼酸盐的特异性拮抗剂，它降低了微生物的生长速率和 2-糠酸的消耗，但对琥珀酸盐的生长没有影响。相应地，当微生物在含有越来越多的钨酸盐的情况下生长在 2-糠酸上时，2-糠酰-CoA 脱氢酶的活性降低。钼酸盐的添加逆转了对 2-糠酰-CoA 脱氢酶活性的负效应，这表明该反应涉及一种钼酶。研究的两种酶都是可诱导的。在该微生物中未检测到质粒。
