斯达氏油脂酵母葡聚糖酶的诱导
Induction of Lipomyces starkeyi Dextranase.
作者信息
Koenig David W, Day Donal F
机构信息
Department of Microbiology, Louisiana State University, and The Audubon Sugar Institute, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803.
出版信息
Appl Environ Microbiol. 1989 Aug;55(8):2079-2081. doi: 10.1128/aem.55.8.2079-2081.1989.
Lipomyces starkeyi ATCC 20825 is a derepressed mutant derived from L. starkeyi ATCC 12659. It requires the presence of an inducer before it produces dextranase. This study was undertaken to determine the most efficient, commercially feasible method for inducing this enzyme. The following compounds induced dextranase synthesis: 1-O-beta-methyl-glucopyranoside, 1-O-alpha-methyl-glucopyranoside, dextran, isomaltopentose, isomaltotetraose, isomaltotriose, and isomaltose. 1-O-beta-Methyl-glucopyranoside was found to be a gratuitous inducer. Early in the growth phase, cells produced higher specific levels of enzyme than they did in late log phase. The length of exposure of the yeast cells to the inducer also affected the amount of dextranase produced. The maximum amount of enzyme was produced after 12 h of exposure to the inducer. The saturation concentration was the same for all inducers tested, i.e., approximately 1 mg of inducer for every 2 x 10 cells.
斯达氏油脂酵母ATCC 20825是由斯达氏油脂酵母ATCC 12659衍生而来的去阻遏突变体。它在产生葡聚糖酶之前需要有诱导剂存在。进行这项研究是为了确定诱导这种酶的最有效且商业上可行的方法。以下化合物可诱导葡聚糖酶的合成：1-O-β-甲基吡喃葡萄糖苷、1-O-α-甲基吡喃葡萄糖苷、葡聚糖、异麦芽五糖、异麦芽四糖、异麦芽三糖和异麦芽糖。发现1-O-β-甲基吡喃葡萄糖苷是一种安慰诱导剂。在生长阶段早期，细胞产生的酶比对数后期的细胞产生的酶具有更高的比活性。酵母细胞暴露于诱导剂的时间长度也会影响所产生的葡聚糖酶的量。暴露于诱导剂12小时后产生的酶量最大。所有测试的诱导剂的饱和浓度相同，即每2×10个细胞约1毫克诱导剂。
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