Kim D, Day D F
Department of Microbiology, Louisiana State University, Baton Rouge.
Enzyme Microb Technol. 1994 Oct;16(10):844-8. doi: 10.1016/0141-0229(94)90058-2.
A mixed-culture fermentation system was designed for the production of size-limited dextrans. This process was simpler and more economical than traditional methods. It required the establishment of microbial consortia of Lipomyces starkeyi ATCC 74054 and Leuconostoc mesenteroides ATCC 10830. Controlling initial conditions, growth, and enzyme production by both organisms controlled the product size. In this process, both strains were grown separately and then mixed. Dextran fermentation was then allowed to proceed. At the desired time (and molecular size), the fermentation was harvested. The optimum pH and temperature for production of clinical dextran (75,000 MW) were 5.2 (+/- 0.1) and 28 (+/- 0.5) degrees C, respectively. Varying the ratio of L. mesenteroides to L. starkeyi in the inoculum did not significantly affect either the final cell ratios or dextran production.
设计了一种混合培养发酵系统用于生产尺寸受限的葡聚糖。该工艺比传统方法更简单、更经济。它需要建立斯达氏油脂酵母ATCC 74054和肠系膜明串珠菌ATCC 10830的微生物群落。通过控制两种微生物的初始条件、生长和酶产生来控制产物大小。在此过程中，两种菌株分别培养然后混合。然后进行葡聚糖发酵。在所需时间（和分子大小）收获发酵产物。生产临床葡聚糖（75,000分子量）的最佳pH值和温度分别为5.2（±0.1）和28（±0.5）摄氏度。改变接种物中肠系膜明串珠菌与斯达氏油脂酵母的比例对最终细胞比例或葡聚糖产量均无显著影响。
