NCIM, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, and Department of Zoology, University of Poona, Pune 411 007, India.
Appl Environ Microbiol. 1989 Oct;55(10):2675-80. doi: 10.1128/aem.55.10.2675-2680.1989.
A stable hybrid obtained by protoplast fusion between a Cellulomonas sp. and Bacillus subtilis exhibits an altered pattern of enzyme induction with different cellulosic substrates. Unlike in the Cellulomonas sp., xylanase was induced in the hybrid organism specifically by xylan, and endoglucanase was induced by carboxymethyl cellulose. The amount and specific activity of xylanase produced by the hybrid were more than those produced by the Cellulomonas sp. beta-Glucosidase which is cell bound or intracellular in the Cellulomonas sp. was secreted by the hybrid organism, and relative amounts of extracellular beta-glucosidase were high. Furthermore, this extracellular beta-glucosidase activity was dependent on the nature of the cellulosic substrate. Endoglucanases synthesized in the hybrid differed in their electrophoretic mobilities as compared with the parental enzymes.
通过纤维素单胞菌和枯草芽孢杆菌原生质体融合获得的稳定杂种，其酶诱导模式与不同的纤维素底物发生了改变。与纤维素单胞菌不同的是，木聚糖酶在杂种中仅由木聚糖诱导，内切葡聚糖酶由羧甲基纤维素诱导。杂种产生的木聚糖酶的量和比活力均高于纤维素单胞菌。纤维素单胞菌中细胞结合或细胞内的β-葡萄糖苷酶由杂种生物分泌，并且细胞外β-葡萄糖苷酶的相对含量较高。此外，这种细胞外β-葡萄糖苷酶活性取决于纤维素底物的性质。与亲本酶相比，杂种中合成的内切葡聚糖酶在电泳迁移率上有所不同。
