Deb J K, Malik S, Ghosh V K, Mathai S, Sethi R
Biochemical Engineering Research Centre, Indian Institute of Technology, New Delhi.
FEMS Microbiol Lett. 1990 Sep 15;59(3):287-92. doi: 10.1016/0378-1097(90)90235-i.
Hybrids between a strain of Bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and Corynebacterium acetoacidophilum, a lysine producer, were prepared by protoplast fusion. Based on distinctive parental biochemical characteristics the fusants were grouped into 9 categories, viz. BC1 through BC9. Three of the hybrids, BC5, BC7a and BC7b, were tested for their ability to produce xylanase and lysine. Both BC7a and BC7b produced xylanase but BC5 did not, however all of them produced lysine albeit to different degrees. These results demonstrate that intergeneric gene transfer takes place through protoplast fusion between these 2 important genera of bacteria and some of the fusants inherit the useful traits of both the parents.
通过原生质体融合制备了我们实验室分离的具有降解木聚糖和其他复杂多糖能力的枯草芽孢杆菌菌株与赖氨酸生产菌嗜酸棒状杆菌之间的杂种。根据亲本独特的生化特性，将融合体分为9类，即BC1至BC9。对其中三个杂种BC5、BC7a和BC7b进行了木聚糖酶和赖氨酸生产能力测试。BC7a和BC7b都产生木聚糖酶，但BC5不产生，然而它们都能产生赖氨酸，尽管程度不同。这些结果表明，属间基因转移通过这两个重要细菌属之间的原生质体融合发生，并且一些融合体继承了双亲的有用性状。
