Williams M N, Klein S, Signer E R
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Appl Environ Microbiol. 1989 Dec;55(12):3229-30. doi: 10.1128/aem.55.12.3229-3230.1989.
A host restriction difference exists between Rhizobium meliloti Rm41 and SU47 exists as indicated by the reduce plating efficiency of transducing phage PhiM12h1. Restriction can be attenuated by incubating cells at 42 degrees C for 3 h; this procedure overcomes a block to transduction from SU47 to Rm41.
如转导噬菌体PhiM12h1的平板接种效率降低所示,苜蓿根瘤菌Rm41和SU47之间存在宿主限制差异。通过将细胞在42摄氏度下孵育3小时可减弱限制作用;此程序克服了从SU47到Rm41转导的障碍。