转译融合两种不同苏云金芽孢杆菌杀虫晶体蛋白基因的产物扩大了杀虫谱。
A Translation Fusion Product of Two Different Insecticidal Crystal Protein Genes of Bacillus thuringiensis Exhibits an Enlarged Insecticidal Spectrum.
机构信息
Stichting Ital, P.O. Box 48, 6700 AA Wageningen, The Netherlands.
出版信息
Appl Environ Microbiol. 1990 Mar;56(3):823-5. doi: 10.1128/aem.56.3.823-825.1990.
Abstract
Two truncated Bacillus thuringiensis crystal protein genes, belonging to the classes cryIA(b) and cryIC and both coding for insecticidal N-terminal fragments of the corresponding crystal proteins, were translationally fused. Expression of the gene fusion in Escherichia coli showed a biologically active protein with a toxicity spectrum that overlapped those of both contributing crystal proteins.
摘要
两个截短的苏云金芽孢杆菌晶体蛋白基因，属于 cryIA(b) 和 cryIC 两类，均编码相应晶体蛋白的杀虫 N 端片段，被翻译融合。该基因融合在大肠杆菌中的表达产生了一种具有生物活性的蛋白质，其毒性谱与两种贡献晶体蛋白的毒性谱重叠。
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