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转译融合两种不同苏云金芽孢杆菌杀虫晶体蛋白基因的产物扩大了杀虫谱。

A Translation Fusion Product of Two Different Insecticidal Crystal Protein Genes of Bacillus thuringiensis Exhibits an Enlarged Insecticidal Spectrum.

机构信息

Stichting Ital, P.O. Box 48, 6700 AA Wageningen, The Netherlands.

出版信息

Appl Environ Microbiol. 1990 Mar;56(3):823-5. doi: 10.1128/aem.56.3.823-825.1990.

DOI:10.1128/aem.56.3.823-825.1990
PMID:16348153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183429/
Abstract

Two truncated Bacillus thuringiensis crystal protein genes, belonging to the classes cryIA(b) and cryIC and both coding for insecticidal N-terminal fragments of the corresponding crystal proteins, were translationally fused. Expression of the gene fusion in Escherichia coli showed a biologically active protein with a toxicity spectrum that overlapped those of both contributing crystal proteins.

摘要

两个截短的苏云金芽孢杆菌晶体蛋白基因，属于 cryIA(b) 和 cryIC 两类，均编码相应晶体蛋白的杀虫 N 端片段，被翻译融合。该基因融合在大肠杆菌中的表达产生了一种具有生物活性的蛋白质，其毒性谱与两种贡献晶体蛋白的毒性谱重叠。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40bf/183429/7fa5da90e1db/aem00068-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40bf/183429/7fa5da90e1db/aem00068-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40bf/183429/7fa5da90e1db/aem00068-0251-a.jpg

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Expression of the cry1EC gene in castor (Ricinus communis L.) confers field resistance to tobacco caterpillar (Spodoptera litura Fabr) and castor semilooper (Achoea janata L.).cry1EC基因在蓖麻（Ricinus communis L.）中的表达赋予了对烟草夜蛾（Spodoptera litura Fabr）和蓖麻半角螟（Achoea janata L.）的田间抗性。
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Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells.重组杆状病毒在昆虫细胞中表达的细菌晶体蛋白的杀虫活性。
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Development of a hybrid delta-endotoxin and its expression in tobacco and cotton for control of a polyphagous pest Spodoptera litura.一种杂交δ-内毒素的研制及其在烟草和棉花中的表达，用于防治多食性害虫斜纹夜蛾。
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Insect-resistant chrysanthemum calluses by introduction of a Bacillus thuringiensis crystal protein gene.通过导入苏云金芽孢杆菌晶体蛋白基因获得抗虫菊花愈伤组织。
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苏云金芽孢杆菌 3 种鳞翅目特异性杀虫晶体蛋白的单克隆抗体分析与杀虫谱
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Nucleotide sequence of crystal protein gene isolated from B. thuringiensis subspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species.从苏云金芽孢杆菌昆虫亚种60.5中分离出的编码对夜蛾属物种具有高活性毒素的晶体蛋白基因的核苷酸序列。
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Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta.苏云金芽孢杆菌库尔斯塔克亚种HD - 73晶体蛋白的全长和截短质粒克隆及其对烟草天蛾的毒性。
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Specificity of Bacillus thuringiensis delta-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts.苏云金芽孢杆菌δ-内毒素的特异性与目标昆虫中肠刷状缘膜中高亲和力结合位点的存在相关。
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Multiplicity of delta-endotoxin genes with different insecticidal specificities in Bacillus thuringiensis aizawai 7.29.苏云金芽孢杆菌库斯塔克亚种7.29中具有不同杀虫特异性的δ-内毒素基因的多样性。
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