平板法检测海藻酸盐裂解酶及其底物特异性分析。
Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity.
机构信息
Department of Biochemistry, University of Wales, P.O. Box 903, Cardiff CF1 1ST, United Kingdom.
出版信息
Appl Environ Microbiol. 1990 Jul;56(7):2265-7. doi: 10.1128/aem.56.7.2265-2267.1990.
Abstract
A plate assay to detect the presence of alginate lyases (EC 4.2.2.3) has been developed. The simultaneous use of specific alginate block structures of defined composition allows the substrate specificity of the enzymes to be determined. Clearing zones in the alginate-containing media are visualized with either cetyl pyridinium chloride or ruthenium red.
摘要
已开发出一种用于检测褐藻胶裂解酶（EC 4.2.2.3）存在的平板测定法。同时使用具有明确定义组成的特定褐藻胶块结构，可以确定酶的底物特异性。在含褐藻酸盐的培养基中用十六烷基吡啶氯或钌红可视化可见的清除区。
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