培养条件对地中海嗜盐嗜碱菌生产聚（β-羟基丁酸）的影响。
Effects of Culture Conditions on Poly(beta-Hydroxybutyric Acid) Production by Haloferax mediterranei.
作者信息
Lillo Jose Garcia, Rodriguez-Valera Francisco
机构信息
Departamento de Genética Molecular y Microbiología, Campus de San Juan, Apdo. 374, Universidad de Alicante, 03080 Alicante, Spain.
出版信息
Appl Environ Microbiol. 1990 Aug;56(8):2517-2521. doi: 10.1128/aem.56.8.2517-2521.1990.
Abstract
The halobacterium Haloferax mediterranei accumulates poly(beta-hydroxybutyrate) (PHB) as intracellular granules. The conditions for PHB production in batch and continuous cultures have been studied and optimized. Phosphate limitation is essential for PHB accumulation in large quantities. Glucose and starch are the best carbon sources. With 2% starch, 0.00375% KH(2)PO(4), and 0.2% NH(4)Cl in batch culture, a production of ca. 6 g of PHB per liter was reached, being 60% of the total biomass dry weight, and giving a yield over the carbon source of 0.33 g/g. The PHB production in continuous cultures was stable over a 3-month period. Our results demonstrate that H. mediterranei is an interesting candidate for industrial production of biological polyesters.
摘要
嗜盐菌地中海富盐菌（Haloferax mediterranei）会将聚（β-羟基丁酸酯）（PHB）积累为细胞内颗粒。已对分批培养和连续培养中PHB的生产条件进行了研究和优化。磷酸盐限制对于大量积累PHB至关重要。葡萄糖和淀粉是最佳碳源。在分批培养中，使用2%淀粉、0.00375% KH₂PO₄和0.2% NH₄Cl时，每升可生产约6克PHB，占总生物量干重的60%，碳源产率为0.33克/克。连续培养中的PHB产量在3个月内保持稳定。我们的结果表明，地中海富盐菌是生物聚酯工业化生产的一个有吸引力的候选菌株。
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