除活性位点外，生淀粉消化但不吸附生淀粉的枯草芽孢杆菌65 α-淀粉酶上亲和位点的出现。
Occurrence of an Affinity Site apart from the Active Site on the Raw-Starch-Digesting but Non-Raw-Starch-Adsorbable Bacillus subtilis 65 alpha-Amylase.
作者信息
Hayashida Shinsaku, Teramoto Yuji, Inoue Takehiro, Mitsuiki Shinji
机构信息
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.
出版信息
Appl Environ Microbiol. 1990 Aug;56(8):2584-2586. doi: 10.1128/aem.56.8.2584-2586.1990.
Abstract
alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-digesting enzymes was proven also with bacterial amylase.
摘要
α-环糊精能特异性抑制枯草芽孢杆菌65 α-淀粉酶对生淀粉的消化作用。当通过蛋白水解作用删除了与生淀粉消化所必需的亲和位点相对应的特定结构域时，该α-淀粉酶的生淀粉消化率和α-环糊精-琼脂糖6B吸附性同时丧失。细菌淀粉酶也证明了生淀粉消化酶上存在亲和位点。
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