Department of Biological Sciences, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931.
Appl Environ Microbiol. 1990 Sep;56(9):2801-6. doi: 10.1128/aem.56.9.2801-2806.1990.
A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utility of this procedure was demonstrated by conducting physiological studies on the influence of CO(2) and FeSO(4) availability on the growth of T. ferrooxidans. In addition, we describe a procedure for the long-term maintenance of cells T. ferrooxidans that ensures culture purity and genetic stability.
一种快速灵敏的分光光度法被开发出来，用于监测液体培养中的嗜酸氧化亚铁硫杆菌的生长。在 500nm 处测定的经洗涤的嗜酸氧化亚铁硫杆菌细胞悬浮液的光密度值与总细胞数和总细胞蛋白浓度成正比，为培养生长速率提供了准确的测量。通过对 CO(2)和 FeSO(4)供应对嗜酸氧化亚铁硫杆菌生长的影响进行生理研究，证明了该程序的实用性。此外，我们还描述了一种长期维持嗜酸氧化亚铁硫杆菌细胞的方法，该方法可确保培养物的纯度和遗传稳定性。
