Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Ontario, Canada K1S 5B6.
Appl Environ Microbiol. 1990 Sep;56(9):2866-9. doi: 10.1128/aem.56.9.2866-2869.1990.
A region of DNA which determined the production of the insecticidal toxin of Bacillus thuringiensis subsp. israelensis was cloned into a derivative of a broad-host-range group IncQ plasmid vector of gram-negative bacteria. The plasmid which we constructed was transferred by conjugative mobilization into a Bradyrhizobium species that nodulates pigeon peas. In this species the construction was maintained stably in the absence of selection and expressed the gene that was installed. Experiments in a greenhouse with the strain which we constructed indicated that this organism provides protection against root nodule damage by the larvae of the insect Rivellia angulata (Diptera).
一段决定苏云金芽孢杆菌亚种 israelensis 杀虫毒素产生的 DNA 区域被克隆到革兰氏阴性菌的广泛宿主范围组 IncQ 质粒载体的衍生物中。我们构建的质粒通过共轭动员转移到根瘤菌属的一种能结瘤兵豆的物种中。在没有选择的情况下，该构建体在这个物种中稳定维持，并表达了所安装的基因。我们构建的菌株在温室中的实验表明，该生物提供了对昆虫 Rivellia angulata（双翅目）幼虫对根瘤损伤的保护。
