Division of Biochemistry, Department of Biological Sciences, The University of Calgary, Calgary, Alberta, Canada T2N 1N4; Nova Husky Research Corporation, Calgary, Alberta, Canada T2E 7K7 ; and Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
Appl Environ Microbiol. 1990 Dec;56(12):3748-54. doi: 10.1128/aem.56.12.3748-3754.1990.
The distribution of genes for [Fe], [NiFe], and [NiFeSe] hydrogenases was determined for 22 Desulfovibrio species. The genes for [NiFe] hydrogenase were present in all species, whereas those for the [Fe] and [NiFeSe] hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio (R. Devereux, M. Delaney, F. Widdel, and D. A. Stahl, J. Bacteriol. 171:6689-6695, 1989) did not react with the [NiFe] hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.
对 22 种脱硫弧菌属的物种进行了[Fe]、[NiFe]和[NiFeSe]氢化酶基因的分布分析。所有物种都存在[NiFe]氢化酶基因，而[Fe]和[NiFeSe]氢化酶基因的分布则较为有限。除脱硫弧菌属（R. Devereux、M. Delaney、F. Widdel 和 D. A. Stahl，J. Bacteriol. 171:6689-6695, 1989）以外的 16S rRNA 组的硫酸盐还原菌不与[NiFe]氢化酶基因探针发生反应，该探针可用于在乳酸盐-硫酸盐培养基上生长后，鉴定油田样品中的不同脱硫弧菌属物种。
