产氢酶基因在脱硫弧菌属中的分布及其在油田环境中物种鉴定中的应用。
Distribution of Hydrogenase Genes in Desulfovibrio spp. and Their Use in Identification of Species from the Oil Field Environment.
机构信息
Division of Biochemistry, Department of Biological Sciences, The University of Calgary, Calgary, Alberta, Canada T2N 1N4; Nova Husky Research Corporation, Calgary, Alberta, Canada T2E 7K7 ; and Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
出版信息
Appl Environ Microbiol. 1990 Dec;56(12):3748-54. doi: 10.1128/aem.56.12.3748-3754.1990.
The distribution of genes for [Fe], [NiFe], and [NiFeSe] hydrogenases was determined for 22 Desulfovibrio species. The genes for [NiFe] hydrogenase were present in all species, whereas those for the [Fe] and [NiFeSe] hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio (R. Devereux, M. Delaney, F. Widdel, and D. A. Stahl, J. Bacteriol. 171:6689-6695, 1989) did not react with the [NiFe] hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.
对 22 种脱硫弧菌属的物种进行了[Fe]、[NiFe]和[NiFeSe]氢化酶基因的分布分析。所有物种都存在[NiFe]氢化酶基因，而[Fe]和[NiFeSe]氢化酶基因的分布则较为有限。除脱硫弧菌属（R. Devereux、M. Delaney、F. Widdel 和 D. A. Stahl，J. Bacteriol. 171:6689-6695, 1989）以外的 16S rRNA 组的硫酸盐还原菌不与[NiFe]氢化酶基因探针发生反应，该探针可用于在乳酸盐-硫酸盐培养基上生长后，鉴定油田样品中的不同脱硫弧菌属物种。
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