Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands, and Station de Recherches Laitieres, Institute National de la Recherche Agronomique, 78350 Jouy-en-Josas, and Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institute National de la Recherche Agronomique, 7850 Thiverval-Grignon, France.
Appl Environ Microbiol. 1992 Jan;58(1):285-90. doi: 10.1128/aem.58.1.285-290.1992.
The localization of two aminopeptidases, an X-prolyl-dipeptidyl aminopeptidase, an endopeptidase, and a tripeptidase in Lactococcus lactis was studied. Polyclonal antibodies raised against each purified peptidase are specific and do not cross-react with other peptidases. Experiments were performed by immunoblotting after cell fractionation and by electron microscopy of immunogold-labeled peptidases. All peptidases were found to be intracellular. However, immunogold studies showed a peripheral labeling of the X-prolyl-dipeptidyl aminopeptidase, the tripeptidase, and the endopeptidase. This peripheral location was further supported by the detection of these three enzymes in cell membrane fractions in which none of the two aminopeptidases was present.
研究了两种氨肽酶（一种 X-脯氨酰二肽酰氨基肽酶、一种内肽酶和一种三肽酶）在乳酸乳球菌中的定位。针对每种纯化的肽酶产生的多克隆抗体是特异性的，不会与其他肽酶发生交叉反应。通过细胞分级分离后的免疫印迹实验和免疫金标记肽酶的电子显微镜实验进行了实验。所有肽酶均被发现存在于细胞内。然而，免疫金研究显示 X-脯氨酰二肽酰氨基肽酶、三肽酶和内肽酶存在外周标记。这种外周位置进一步得到了三种酶在细胞膜部分的检测结果的支持，在该部分不存在两种氨肽酶中的任何一种。
