Departamento de Bioquímica Vegetal, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Apto. 419, 18080 Granada, Spain, and Department of Biochemistry, University of Groningen, Groningen, The Netherlands.
Appl Environ Microbiol. 1992 Jan;58(1):415-7. doi: 10.1128/aem.58.1.415-417.1992.
Expression of the xylMA genes encoding for toluene monoxygenase from the lactose promoter in a broad-host-range plasmid allows the oxidation of toluene and m- and p-nitrotoluene to their corresponding benzyl alcohols and benzaldehydes in Pseudomonas putida and Escherichia coli. Benzyl alcohols accumulate until reaching a concentration of about 80 muM, while benzaldehydes accumulate steadily with time for at least 24 h. TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase recognize m- and p-nitro-substituted compounds as substrates. In contrast, the XylR protein, which regulates the TOL plasmid-encoded upper-pathway operon, does not recognize nitro-substituted toluenes as effectors.
乳糖启动子控制的 xylMA 基因编码的甲苯单加氧酶在一个广谱宿主质粒中的表达使得甲苯以及间位和对位硝基甲苯能够在恶臭假单胞菌和大肠杆菌中被氧化为相应的苄醇和苯甲醛。苄醇积累到大约 80μM 时达到积累顶峰,而苯甲醛则在 24 小时内随着时间的推移稳定积累。TOL 编码的苄醇脱氢酶和苯甲醛脱氢酶能够识别间位和对位硝基取代的化合物作为底物。相比之下,调节 TOL 质粒编码的上游途径操纵子的 XylR 蛋白不能将硝基取代的甲苯识别为效应物。