恶臭假单胞菌（Pseudomonas (putida (arvilla) mt-2）对甲苯和二甲苯的代谢：TOL 质粒新功能的证据
Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.
作者信息
Worsey M J, Williams P A
出版信息
J Bacteriol. 1975 Oct;124(1):7-13. doi: 10.1128/jb.124.1.7-13.1975.
Abstract
Pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m-xylene, and p-xylene on a transmissible plasmid, TOL. These compounds are degraded by oxidation of one of the methyl substituents via the corresponding alcohols and aldehydes to benzoate and m- and p-toluates, respectively, which are then further metabolised by the meta pathway, also coded for by the TOL plasmid. The specificities of the benzyl alcohol dehydrogenase and the benzaldehyde dehydrogenase for their three respective substrates are independent of the carbon source used for growth, suggesting that a single set of nonspecific enzymes is responsible for the dissimilation of the breakdown products of toluene and m- and p-xylene. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase are coincidently and possible coordinately induced by toluene and the xylenes, and by the corresponding alcohols and aldehydes. They are not induced in cells grown on m-toluate but catechol 2,3-oxygenase can be induced by m-xylene.
摘要
恶臭假单胞菌（阿维拉）mt-2在可转移质粒TOL上携带甲苯、间二甲苯和对二甲苯分解代谢的基因。这些化合物通过甲基取代基之一经相应的醇和醛氧化分别生成苯甲酸、间甲苯酸和对甲苯酸，然后通过也由TOL质粒编码的间位途径进一步代谢。苄醇脱氢酶和苯甲醛脱氢酶对其三种各自底物的特异性与用于生长的碳源无关，这表明一组单一的非特异性酶负责甲苯、间二甲苯和对二甲苯分解产物的异化作用。苄醇脱氢酶和苯甲醛脱氢酶同时且可能协同地由甲苯、二甲苯以及相应的醇和醛诱导。它们在以间甲苯酸生长的细胞中不被诱导，但儿茶酚2,3-加氧酶可由间二甲苯诱导。
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