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对苯二胺在显色检测儿茶酚和原儿茶酸中的应用，儿茶酚和原儿茶酸是芳香族化合物代谢中的二酚中间产物。

Application of p-Toluidine in Chromogenic Detection of Catechol and Protocatechuate, Diphenolic Intermediates in Catabolism of Aromatic Compounds.

机构信息

Department of Biology, Yale University, P.O. Box 6666, New Haven, Connecticut 06511-8112.

出版信息

Appl Environ Microbiol. 1992 Aug;58(8):2694-7. doi: 10.1128/aem.58.8.2694-2697.1992.

DOI:10.1128/aem.58.8.2694-2697.1992
PMID:16348758
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC195844/
Abstract

In the presence of p-toluidine and iron, protocatechuate and catechols yield color. Inclusion of p-toluidine in media facilitates the screening of microbial strains for alterations affecting aromatic catabolism. Such strains include mutants affected in the expression of oxygenases and Escherichia coli colonies carrying cloned or subcloned aromatic catabolic genes which encode enzymes giving rise to protocatechuate or catechol. The diphenolic detection system can also be applied to the creation of vectors relying on insertion of cloned DNA into one of the latter marker genes.

摘要

在对甲苯胺和铁的存在下，原儿茶酸和儿茶酚显色。在培养基中加入对甲苯胺有助于筛选影响芳香族分解代谢的微生物菌株。这些菌株包括影响加氧酶表达的突变体和携带芳香族分解代谢基因克隆或亚克隆的大肠杆菌菌落，这些基因编码导致原儿茶酸或儿茶酚的酶。该二酚检测系统也可用于依赖于将克隆 DNA 插入后者的一个标记基因之一的载体的创建。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/430c/195844/ce2d488e0e7a/aem00049-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/430c/195844/d51bb8485e81/aem00049-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/430c/195844/ce2d488e0e7a/aem00049-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/430c/195844/d51bb8485e81/aem00049-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/430c/195844/ce2d488e0e7a/aem00049-0364-a.jpg

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