用于木聚糖酶浊度法测定的稳定定义底物。
Stable defined substrate for turbidimetric assay of endoxylanases.
机构信息
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, Manchester M60 1QD, United Kingdom.
出版信息
Appl Environ Microbiol. 1992 Oct;58(10):3433-6. doi: 10.1128/aem.58.10.3433-3436.1992.
A stable xylan suspension was prepared and characterized. Hydrolysis of the particles converts them into soluble fragments, thereby lowering the turbidity of the suspension. The small volume of the assay mixture, the short incubation time required, and the simplicity of the procedure permit the rapid analysis of many samples. Furthermore, the procedure can be used to assay xylanase activities in the presence of other reducing materials and is also useful for monitoring low-level xylanase activities.
制备并表征了一种稳定的木聚糖悬浮液。颗粒的水解将其转化为可溶性片段，从而降低了悬浮液的浊度。该测定方法的混合物体积小，所需的孵育时间短，且操作简单，可快速分析大量样品。此外，该方法可用于在存在其他还原物质的情况下测定木聚糖酶活性，也可用于监测低水平的木聚糖酶活性。
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