从苜蓿根际土壤中分离根瘤菌 meliloti 结瘤基因诱导剂。
Isolation of Rhizobium meliloti nod Gene Inducers from Alfalfa Rhizosphere Soil.
机构信息
Department of Agronomy and Range Science, University of California, Davis, California 95616.
出版信息
Appl Environ Microbiol. 1993 Feb;59(2):636-9. doi: 10.1128/aem.59.2.636-639.1993.
Abstract
Methanolic extracts of alfalfa rhizosphere soil induce more nod gene transcription in Rhizobium meliloti than extracts of nonrhizosphere soil. Six peaks of nod-inducing activity were separated by high-performance liquid chromatography from rhizosphere soil extract, and one compound was identified by H nuclear magnetic resonance, mass spectrometry, and UV-visible spectra as a formononetin-7-O-glycoside that activates both NodD1 and NodD2 proteins. The unanticipated presence of a glycoside in rhizosphere soil suggests either that large amounts of the glycoside were exuded by roots or that some glycosides are unexpectedly stable in soil.
摘要
苜蓿根际土壤的甲醇提取物比非根际土壤的提取物能诱导更多的根瘤菌 meliloti 基因转录。从根际土壤提取物中通过高效液相色谱分离出 6 个诱导活性峰，通过氢核磁共振、质谱和紫外可见光谱鉴定出一种芒柄花素-7-O-糖苷，它能激活 NodD1 和 NodD2 蛋白。根际土壤中糖苷的意外存在表明，要么是大量糖苷被根系分泌出来，要么是某些糖苷在土壤中出人意料地稳定。
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