Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan Street, Albany, California 94710.
Appl Environ Microbiol. 1993 Apr;59(4):1054-7. doi: 10.1128/aem.59.4.1054-1057.1993.
The triacylglycerol (TG) profiles of teliospores of Tilletia controversa and Tilletia tritici were examined by high-performance liquid chromatography (HPLC) and gas-liquid chromatography. Boiling isopropanol was used to ensure enzyme inactivation during homogenization. The largest lipid component as determined by thin-layer chromatography was TGs. On the basis of thin-layer chromatography of crude lipid extracts, T. controversa and T. tritici do not contain a large amount of free fatty acids. TG profiles of T. controversa and T. tritici were very similar, with 18 species of TGs resolved by HPLC and gas-liquid chromatography. In both organisms, PLL (palmitic, linoleic, linoleic) was the major component, followed by LLL (trilinolein) and PLO (palmitic, linoleic, oleic). The ratio of PLO to PLL was 1:6 and 1:4 in T. tritici and T. controversa, respectively. The TGs of both organisms contain long-chain (>22 carbons) mono- and dienoic acids. Linoleic acid was the major fatty acid found in TGs from both organisms. The differences of TGs were not considered significant for differentiation purposes.
采用高效液相色谱法（HPLC）和气相色谱法分析了黑粉菌属的厚垣孢子和小麦坚黑粉菌的三酰基甘油（TG）图谱。在匀浆过程中使用沸腾异丙醇以确保酶失活。通过薄层色谱法确定最大的脂质组分为 TG。根据粗脂质提取物的薄层色谱分析，黑粉菌属和小麦坚黑粉菌不含大量游离脂肪酸。黑粉菌属和小麦坚黑粉菌的 TG 图谱非常相似，通过 HPLC 和气相色谱法可分辨出 18 种 TG。在这两种生物中，PLL（棕榈酸、亚油酸、亚油酸）是主要成分，其次是 LLL（三亚油酸酯）和 PLO（棕榈酸、亚油酸、油酸）。PLO 与 PLL 的比例分别为 1:6 和 1:4，在小麦坚黑粉菌和黑粉菌属中。两种生物的 TG 都含有长链（>22 个碳原子）单烯和二烯酸。亚油酸是两种生物 TG 中主要的脂肪酸。TG 的差异不认为对分类有重要意义。
