Department of Botany, Stockholm University, S-106 91 Stockholm, Sweden; Marine Science Research Center, State University of New York at Stony Brook, Stony Brook, New York 11794-5000 ; and Biophysical Chemistry Group, Institute of Physical Chemistry, University of Vienna, Vienna, Austria.
Appl Environ Microbiol. 1993 Oct;59(10):3239-44. doi: 10.1128/aem.59.10.3239-3244.1993.
Immunochemical labeling was used to study the subcellular distribution of cytochrome oxidase, a respiratory protein, in Trichodesmium thiebautii. The protein was found associated with both cytoplasmic and thylakoid membranes. About a sixfold variation in the protein content (gold particle count) was found among Trichodesmium cells within a single colony. Double labeling was performed with cytochrome oxidase and nitrogenase antisera. Regression analysis of gold particle counts per unit of cell area of cytochrome oxidase and nitrogenase showed a positive correlation (r = 0.911); cells with higher nitrogenase levels also had higher levels of cytochrome oxidase. The parallel expression of two proteins suggests that respiratory oxygen uptake may be involved in nitrogenase protection (respiratory protection) in Trichodesmium spp.
免疫化学标记被用于研究三碘甲烷菌中细胞色素氧化酶（一种呼吸蛋白）的亚细胞分布。该蛋白与细胞质膜和类囊体膜都有关联。在同一个群体的三碘甲烷菌细胞中，发现该蛋白的含量（金颗粒计数）存在约六倍的差异。用细胞色素氧化酶和固氮酶抗血清进行双重标记。细胞色素氧化酶和固氮酶的单位细胞面积的金颗粒计数的回归分析显示出正相关（r = 0.911）；具有更高固氮酶水平的细胞也具有更高水平的细胞色素氧化酶。两种蛋白的平行表达表明，呼吸氧气摄取可能参与三碘甲烷菌属的固氮酶保护（呼吸保护）。
