Center for Biological Resource Recovery and Departments of Microbiology and Biochemistry, University of Georgia, Athens, Georgia 30602.
Appl Environ Microbiol. 1993 Oct;59(10):3498-501. doi: 10.1128/aem.59.10.3498-3501.1993.
Autolysis was induced to form stable, cell wall-free cells of Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200, using a complex medium containing glycine (0.4% wt/vol) and/or sucrose or glycerol (10% wt/vol) at an optimum temperature of 64 degrees C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled form during prolonged incubation. The removal of the cell wall was confirmed by electron microscopy.
采用含有甘氨酸(0.4wt/vol)和/或蔗糖或甘油(10wt/vol)的复杂培养基,在 64°C 的最佳温度下,诱导产热脱硫梭菌 JW102 和嗜热乙醇杆菌 JW200 形成稳定的、无细胞壁的细胞。这两种细菌的原生质体在固体培养基上作为 L 相菌落生长;在长时间孵育过程中,超过 50%的这些菌落再生为有壁形式。细胞壁的去除通过电子显微镜得到确认。