乳球菌指数生长的最小要求。
Minimal Requirements for Exponential Growth of Lactococcus lactis.
机构信息
Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands.
出版信息
Appl Environ Microbiol. 1993 Dec;59(12):4363-6. doi: 10.1128/aem.59.12.4363-4366.1993.
Abstract
A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h. With 19 amino acids added, the growth rate increased to mu = 0.7 h and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
摘要
一种最小生长培养基，含有葡萄糖、醋酸盐、维生素和 8 种氨基酸，允许乳球菌乳亚种生长，在分批培养中的比生长速率为 μ=0.3 h。添加 19 种氨基酸后，生长速率增加到 μ=0.7 h，并且指数生长阶段继续进行，直到达到高细胞浓度。我们表明，3-（N-吗啉）丙磺酸（MOPS）是乳球菌的合适缓冲液，并且可以在高浓度下使用。
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