Mossberg K A, Taegtmeyer H
Department of Medicine, University of Texas Health Science Center, Houston.
J Nucl Med. 1992 Aug;33(8):1523-9.
Since skeletal muscle has been implicated as the major site of insulin resistance, the purpose of this study was to examine in detail the time course of muscle glucose uptake during the onset and maintenance of euglycemic hyperinsulinemia. Uptake of 18F-2-deoxy-2-fluoro-D-glucose (FDG) by the thigh muscle of an anesthetized rabbit was monitored by a single pair of coincidence photon detectors. Graphical analysis of tissue and plasma radioactivity concentrations was performed to derive fractional rates of FDG phosphorylation continuously. FDG phosphorylation rates were determined during rest (glucose 7 mM, insulin 5-10 microU/ml) and subsequent 5-min intervals under conditions of euglycemic hyperinsulinemia (glucose 6-8 mM; insulin 350-400 microU/ml plasma). FDG phosphorylation did not increase above resting control levels until 5.5 +/- 1.5 min after intravenous insulin administration. After 20-30 min of hyperinsulinemia, FDG phosphorylation and calculated glucose metabolic rates were increased by 50%. At 35-40 min of the clamp in place, there was a second increase in tracer phosphorylation which plateaued at 200% of control (p less than 0.01) and remained at this level for the remainder of the experiment. In conclusion, we have described a method for making rapid, serial estimates of insulin-mediated skeletal muscle glucose uptake. We suggest that appraisal of the time course of glucose uptake with FDG will aid in the understanding of normal and pathophysiologic states of insulin action in vivo.
由于骨骼肌被认为是胰岛素抵抗的主要部位，本研究的目的是详细研究正常血糖高胰岛素血症发生和维持期间肌肉葡萄糖摄取的时间进程。通过一对符合光子探测器监测麻醉兔大腿肌肉对18F-2-脱氧-2-氟-D-葡萄糖（FDG）的摄取。对组织和血浆放射性浓度进行图形分析，以连续得出FDG磷酸化的分数率。在静息状态（葡萄糖7 mM，胰岛素5-10微单位/毫升）以及正常血糖高胰岛素血症条件下（葡萄糖6-8 mM；血浆胰岛素350-400微单位/毫升）随后的5分钟间隔内测定FDG磷酸化率。静脉注射胰岛素后5.5±1.5分钟，FDG磷酸化才升高至高于静息对照水平。高胰岛素血症20-30分钟后，FDG磷酸化和计算出的葡萄糖代谢率增加了50%。在钳夹35-40分钟时，示踪剂磷酸化出现第二次升高，达到对照水平的200%（p<0.01），并在实验剩余时间保持在该水平。总之，我们描述了一种快速、连续估计胰岛素介导的骨骼肌葡萄糖摄取的方法。我们认为，用FDG评估葡萄糖摄取的时间进程将有助于理解体内胰岛素作用的正常和病理生理状态。
