Departamento de Bioquímica y Biología Molecular, and Departamento de Microbiología y Biología Celular, Universidad de La Laguna, 38071 Tenerife, Spain.
Appl Environ Microbiol. 1998 Mar;64(3):902-6. doi: 10.1128/AEM.64.3.902-906.1998.
To develop the use of electrophoretic lipopolysaccharide profiles for Bradyrhizobium strain identification, we studied the feasibility of using electrophoresis of whole legume nodule homogenates to obtain distinctive lipopolysaccharide profiles. The electrophoretic patterns were the same whether we used nodule extracts, bacteroids, or cultured bacteria as samples, and there was no evidence of changes in the ladder-like pattern during the nodulation process. To assess the reliability of using lipopolysaccharide profiling performed with individual nodules for studying the diversity and microdistribution of the rhizobia nodulating wild shrub legumes, we used a population of Adenocarpus foliolosus seedlings. We obtained 75 different profiles from the 147 nodules studied. There was no dominant profile in the sample, and a plant with different nodules generally produced different profiles. Electrophoresis of legume root nodules proved to be a fast and discriminating technique for determining the diversity of a bradyrhizobial population, although it did not allow the genetic relationships among the nodulating strains to be studied.
为了开发电泳脂多糖图谱在根瘤菌菌株鉴定中的应用，我们研究了使用整个豆科植物根瘤匀浆电泳获得独特脂多糖图谱的可行性。无论我们使用根瘤提取物、类菌体还是培养细菌作为样品，电泳图谱都是相同的，而且在结瘤过程中没有梯状模式变化的证据。为了评估使用单个根瘤进行脂多糖图谱分析来研究野生灌木豆科植物根瘤菌多样性和微分布的可靠性，我们使用了一批腺果藤幼苗。我们从研究的 147 个根瘤中获得了 75 种不同的图谱。样本中没有优势图谱，而且具有不同根瘤的植物通常会产生不同的图谱。豆科植物根瘤的电泳证明是一种快速且有区别的技术，可用于确定根瘤菌种群的多样性，尽管它不允许研究结瘤菌株之间的遗传关系。
