Emiliani E, de Davie I U
Departamento de Microbiología, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Sante Fe, Argentina.
Appl Microbiol. 1962 Nov;10(6):504-12. doi: 10.1128/am.10.6.504-512.1962.
The examination of substances formed during induced autolysis by Aspergillus niger was continued in this work, which dealt in particular with carbohydrates. The autolysate contained a large amount of d-glucose (14 to 20% dry wt) and traces of glycolic aldehyde, dihydroxyacetone, ribose, xylose, and fructose. It also contained glycopeptides (about 10% dry wt), which were split from the cell wall during autolysis and which differed from one another in their level of polymerization and their composition. They were constituted by glucose and mannose, glucose and galactose, or mannose, glucose, and galactose (mannose being the most abundant in this case), and amino acids (chiefly alanine, serine, glutamic acid, and aspartic acid). During autolysis, only a part of the cell wall was dissolved, since it retained its shape. Upon further chemical hydrolysis, it produced mostly glucose and glucosamine, and smaller amounts of mannose, galactose, and amino acids. Presumably, glucomannoproteins and glucogalactoproteins were present in the intact cell as a macromolecular complex, constituting, together with chitin, the major part of the cell wall of Aspergillus.
本研究继续对黑曲霉诱导自溶过程中形成的物质进行检测，尤其关注碳水化合物。自溶产物含有大量的d-葡萄糖（占干重的14%至20%）以及痕量的乙醇醛、二羟基丙酮、核糖、木糖和果糖。它还含有糖肽（约占干重的10%），这些糖肽在自溶过程中从细胞壁上裂解下来，其聚合程度和组成各不相同。它们由葡萄糖和甘露糖、葡萄糖和半乳糖，或甘露糖、葡萄糖和半乳糖（在这种情况下甘露糖含量最高）以及氨基酸（主要是丙氨酸、丝氨酸、谷氨酸和天冬氨酸）组成。在自溶过程中，只有部分细胞壁溶解，因为它仍保持其形状。进一步化学水解后，它主要产生葡萄糖和氨基葡萄糖，以及少量的甘露糖、半乳糖和氨基酸。据推测，完整细胞中存在葡糖甘露糖蛋白和葡糖半乳糖蛋白作为大分子复合物，与几丁质一起构成黑曲霉细胞壁的主要部分。
