Bahn A N, Kung P C, Hayashi J A
J Bacteriol. 1966 May;91(5):1672-6. doi: 10.1128/jb.91.5.1672-1676.1966.
Bahn, Arthur N. (Northwestern University, Chicago, Ill.), Patrick C. Y. Kung, and James A. Hayashi. Chemical composition and serological analysis of the cell wall of Peptostreptococcus. J. Bacteriol. 91:1672-1676. 1966.-Chemical and serological analyses were made of the cell wall of Peptostreptococcus to characterize taxonomically this genus of anaerobic streptococci. Cell wall hydrolysates of P. putridus strains 06 and 85, P. intermedius strains 11 and 87, and P. elsdenii strain B-159 were prepared, and the cell wall sugars were measured quantitatively by paper chromatography. Strain 85 contained only glucose, whereas strain 06 contained 93% glucose and 7% mannose. Strain 87 contained only rhamnose, and strain 11 contained approximately equal amounts of glucose and rhamnose. Strain B-159 differed from all the other strains in having a low (3.1%) content of total carbohydrate, consisting of rhamnose, galactose, and glucose. Quantitative amino acid analyses showed that the major amino compounds present in the cell wall were glutamic and aspartic acids, alanine, lysine, muramic acid, glucosamine, and galactosamine. Strains 06 and 85 possessed this complement of amino compounds, but strains 11 and 87 had relatively little aspartic acid. Strain B-159 was markedly different in having a high content of glycine and diaminopimelic acid, with only traces of lysine; it was the only strain in which teichoic acid was found. Serological analyses were made with the use of cell wall extracts as antigenic material and with homologous antisera, as well as streptococcal group antisera for groups A through S. The only strong agglutination was obtained between strain 87 antigen and group C antisera; weak agglutination was obtained with 87 against N, O, and K, and between strain 11 and groups E and F. All other antisera gave negative reactions. It is concluded that strain B-159 does not belong to the genus Peptostreptococcus, that strains 06 and 85 are members of P. putridus, and that strains 11 and 87 may be members of two different genera.
巴恩，亚瑟·N.（西北大学，伊利诺伊州芝加哥），帕特里克·C.Y.孔，以及詹姆斯·A.林。消化链球菌细胞壁的化学成分及血清学分析。《细菌学杂志》91:1672 - 1676。1966年。——对消化链球菌的细胞壁进行了化学和血清学分析，以便从分类学角度对这一厌氧链球菌属进行特征描述。制备了腐败消化链球菌菌株06和85、中间消化链球菌菌株11和87以及埃尔氏消化链球菌菌株B - 159的细胞壁水解物，并通过纸色谱法定量测定了细胞壁糖类。菌株85仅含葡萄糖，而菌株06含93%的葡萄糖和7%的甘露糖。菌株87仅含鼠李糖，菌株11含大致等量的葡萄糖和鼠李糖。菌株B - 159与所有其他菌株不同，其总碳水化合物含量低（3.1%），由鼠李糖、半乳糖和葡萄糖组成。定量氨基酸分析表明，细胞壁中存在的主要氨基化合物为谷氨酸、天冬氨酸、丙氨酸、赖氨酸、胞壁酸、葡糖胺和半乳糖胺。菌株06和85具有这种氨基化合物组合，但菌株11和87的天冬氨酸相对较少。菌株B - 159明显不同，其甘氨酸和二氨基庚二酸含量高，仅含微量赖氨酸；它是唯一发现磷壁酸的菌株。使用细胞壁提取物作为抗原物质并结合同源抗血清以及A至S组链球菌抗血清进行了血清学分析。仅在菌株87抗原与C组抗血清之间获得了强凝集反应；菌株87与N、O和K组以及菌株11与E和F组之间获得了弱凝集反应。所有其他抗血清均呈阴性反应。得出的结论是，菌株B - 159不属于消化链球菌属，菌株06和85是腐败消化链球菌的成员，菌株11和87可能属于两个不同的属。
