急性1,2 - 二氯乙烷中毒和烟酰胺给药后大鼠脑突触体组分中的[钠钾 - 三磷酸腺苷酶活性和5 - 羟色胺转运]

[Na+, K+-ATPase activity and serotonin transport in the rat brain synaptosomes fractions after acute 1,2-dichloroethane intoxication and nicotinamide administration].

作者信息

Gubs'kyĭ Iu I, Ianits'ka L V, Velykyĭ M M, Kuchmerovs'ka T M

出版信息

Ukr Biokhim Zh (1999). 2004 Nov-Dec;76(6):106-10.

Abstract

Alterations of Na+,K+-ATPase activity and serotoninergic system functioning were investigated in brain synaptosomes fractions of rats under experimental acute 1,2-dichloroethane (DChE) intoxication. It was shown that Na+,K+-ATPase activity was markedly increased (by 41,8%) in a period of 24 h after DChE intoxication and decreased (by 27%) after 48 h intoxication. The level of [2-14C]-serotonin uptake by synaptosomes was progressively diminished after 24 and 48 h after DChE injection whereas the activity of monoamine uptake proved to be unchanged. Nicotinamide (200 mg/kg of body weight) was administered to rats subjected to DChE 1, 24 and 36 h after poisoning. The treatment of rats with nicotinamide resulted in some normalization of brain synaptosomal Na+, K+-ATPase activity and serotonin uptake controlled at 48 h after DChE intoxication.

摘要

在实验性急性1,2 - 二氯乙烷（DChE）中毒的大鼠脑突触体组分中，研究了Na⁺,K⁺ - ATP酶活性和5 - 羟色胺能系统功能的改变。结果显示，DChE中毒后24小时内，Na⁺,K⁺ - ATP酶活性显著增加（增加41.8%），而中毒48小时后活性降低（降低27%）。DChE注射后24小时和48小时，突触体对[2 - ¹⁴C] - 5 - 羟色胺的摄取水平逐渐降低，而单胺摄取活性则保持不变。在中毒后1小时、24小时和36小时，给遭受DChE中毒的大鼠注射烟酰胺（200毫克/千克体重）。用烟酰胺治疗大鼠后，在DChE中毒48小时时检测发现，脑突触体Na⁺,K⁺ - ATP酶活性和5 - 羟色胺摄取出现了一定程度的恢复正常。