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使用固定化乙醇氧化酶的酒精检测生物传感方法。

Biosensing approach for alcohol determination using immobilized alcohol oxidase.

作者信息

Yildiz Huseyin Bekir, Toppare Levent

机构信息

Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.

出版信息

Biosens Bioelectron. 2006 Jun 15;21(12):2306-10. doi: 10.1016/j.bios.2005.11.006. Epub 2005 Dec 13.

Abstract

Alcohol oxidase (AOD) was immobilized in polypyrrole (PPy) and a random copolymer containing 3-methylthienyl methacrylate and p-vinylbenzyloxy poly(ethyleneoxide) matrices. Immobilization of enzyme was performed via entrapment in conducting polymers during electrochemical polymerization of pyrrole through the thiophene moiety of the copolymer. Three different alcohols, namely methanol, ethanol and n-propanol, were used as substrates. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values, operational stabilities and shelf life of the enzyme electrodes were investigated.

摘要

酒精氧化酶(AOD)被固定在聚吡咯(PPy)以及一种包含甲基丙烯酸3-甲基噻吩酯和对乙烯基苄氧基聚环氧乙烷的无规共聚物基质中。酶的固定是通过在吡咯的电化学聚合过程中,利用共聚物的噻吩部分将酶包埋在导电聚合物中来实现的。使用了三种不同的醇类,即甲醇、乙醇和正丙醇作为底物。研究了酶电极的最大反应速率、米氏常数、最适温度和pH值、操作稳定性以及保质期。

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