Özdemir Merve, Arslan Fatma, Arslan Halit
Department of Chemistry, Faculty of Arts and Sciences, Gazi University, Ankara, Turkey.
Artif Cells Blood Substit Immobil Biotechnol. 2012 Aug;40(4):280-4. doi: 10.3109/10731199.2011.646411. Epub 2012 Apr 3.
In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 °C, respectively. There are two linear parts in the region between 1.0 × 10 (-7) - 1.0 × 10 (-6)M (R(2) = 0.997) and 1.0 × 10 (-5) - 1.0 × 10 (-3) M (R(2) = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
本文通过包埋技术制备了一种新型的安培型胆碱生物传感器，该传感器将胆碱氧化酶固定在电化学聚合的聚吡咯-聚乙烯磺酸盐（PPy-PVS）膜上。研究了pH值和温度的影响，发现最佳参数分别为9.0和60℃。在1.0×10（-7）-1.0×10（-6）M（R（2）=0.997）和1.0×10（-5）-1.0×10（-3）M（R（2）=0.986）范围内有两个线性部分。还研究了酶电极的储存稳定性和操作稳定性。
