Tankson J D, Fedorka-Cray P J, Jackson C R, Headrick M
USDA/Agricultural Research Services, Bacterial and Epidemiology Antimicrobial Resistance Research Unit, 950 College Station Road, Athens, GA 30605, USA.
J Antimicrob Chemother. 2006 Feb;57(2):190-8. doi: 10.1093/jac/dki439. Epub 2005 Dec 13.
In the United States, Salmonella enterica serotype Niakhar is infrequently isolated. Between 1997 and 2000, the animal arm of the National Antimicrobial Resistance Monitoring System-Enteric Bacteria (NARMS) assayed a total of 22,383 Salmonella isolates from various animal sources (swine, cattle, chickens, turkeys, cats, horses, exotics and dogs) for antimicrobial susceptibility. Isolates originated from diagnostic and non-diagnostic submissions.
To study the phenotypic and genotypic characteristics of Salmonella Niakhar.
Only five (0.02%) of the 22,383 isolates were identified as Salmonella Niakhar. Antimicrobial resistance testing indicated that three isolates were pan-susceptible, one isolate was resistant to ampicillin and one isolate was resistant to ampicillin, chloramphenicol, ciprofloxacin, kanamycin, nalidixic acid, streptomycin, sulfamethoxazole, tetracycline and trimethoprim/sulfamethoxazole. RAPD-PCR analysis, PFGE and ribotyping indicated that two pan-susceptible isolates were genetically similar, whereas the three remaining isolates were genetically different. The one Salmonella Niakhar isolate that was multiresistant harboured a class I integron, intI1 and two large plasmids.
This study represents the first report of a ciprofloxacin-resistant Salmonella isolate from the animal arm of NARMS.
在美国，肠炎沙门氏菌尼亚哈尔血清型很少被分离出来。1997年至2000年期间，国家抗微生物药物耐药性监测系统-肠道细菌（NARMS）的动物监测部门共检测了22383株来自各种动物源（猪、牛、鸡、火鸡、猫、马、外来动物和狗）的沙门氏菌分离株的抗微生物药物敏感性。分离株来自诊断性和非诊断性送检样本。
研究沙门氏菌尼亚哈尔的表型和基因型特征。
在22383株分离株中，只有5株（0.02%）被鉴定为沙门氏菌尼亚哈尔。抗微生物药物耐药性检测表明，3株分离株对所有检测药物敏感，1株对氨苄西林耐药，1株对氨苄西林、氯霉素、环丙沙星、卡那霉素、萘啶酸、链霉素、磺胺甲恶唑、四环素和甲氧苄啶/磺胺甲恶唑耐药。随机扩增多态性DNA-PCR（RAPD-PCR）分析、脉冲场凝胶电泳（PFGE）和核糖体分型表明，2株对所有检测药物敏感的分离株在基因上相似，而其余3株分离株在基因上不同。1株多重耐药的沙门氏菌尼亚哈尔分离株携带1个I类整合子、intI1和2个大质粒。
本研究是NARMS动物监测部门首次报告分离出对环丙沙星耐药的沙门氏菌分离株。
