Sanz-Casla M T, Maestro M L, Vidaurreta M, Maestro C, Arroyo M, Cerdán J
Servicio de Análisis Clínicos, Sección Biología Tumoral, Hospital Clínico San Carlos, Madrid, Spain.
Dig Dis. 2005;23(2):151-5. doi: 10.1159/000088597.
Methylation of the CpG islands in the p16 gene promoter region is an important transcription repression mechanism that has been identified as an alternative mechanism for inactivating specific genes in cancer. Given that, DNA methylation is a common event in colorectal cancer.
The aim of this study was to establish the methylation status of the p16 gene in 104 patients with colorectal carcinoma and evaluate its prognostic value. DNA was bisulfite-modified and analyzed for p16 promoter methylation by methylation-specific PCR.
Methylation of thep16 gene was determined in 18.3% of our patient population (19/104). The methylated state did not correlate with any clinicopathological factors. During a median follow-up period of 72 months, the overall survival rate for patients with gene methylation was 75% and without gene methylation it was 61% (p = 0.09).
Although not statistically significant, this difference indicates a clinically valuable tendency.
p16基因启动子区域CpG岛的甲基化是一种重要的转录抑制机制，已被确定为癌症中特定基因失活的一种替代机制。鉴于此，DNA甲基化在结直肠癌中是一种常见现象。
本研究的目的是确定104例结直肠癌患者中p16基因的甲基化状态，并评估其预后价值。对DNA进行亚硫酸氢盐修饰，通过甲基化特异性PCR分析p16启动子甲基化情况。
在我们的患者群体中，18.3%（19/104）的患者检测到p16基因甲基化。甲基化状态与任何临床病理因素均无相关性。在中位随访期72个月期间，基因甲基化患者的总生存率为75%，未发生基因甲基化的患者总生存率为61%（p = 0.09）。
尽管差异无统计学意义，但这种差异表明了一种具有临床价值的趋势。
