Jia Jing, Lu Rong, Zhou Chunlei, Zhao Lan, Fu Zheng, Zhu Zhitong, Wang Li, Zheng Minna, Yang Jing, Zhang Yanjun, Yao Zhi
Department of Immunology, Tianjin Medical University, Tianjin, China.
Mol Carcinog. 2006 Mar;45(3):197-203. doi: 10.1002/mc.20172.
Tyroservatide (YSV) is a bioactive tripeptide of tyrosyl-seryl-valine. In this study, we studied the effects of YSV on human hepatocarcinoma BEL-7402 tumors transplanted in BALB/c (nu/nu) nude mice, and gene expression in the tumor cells with gene-chip analysis. Results show that YSV significantly inhibits the growth of transplanted human hepatocarcinoma BEL-7402 in nude mice (n = 12) compared with the control group (P < 0.05); with an inhibition rate of 55% at 320 microg/kg/d. Seven hundred eighty-one genes were different between the YSV group and the control group. Fifty-two genes changed in expression level by onefold or more including 37 downregulated genes and 15 upregulated genes. Probably, YSV exhibits a significant antitumor activity by inhibiting the expression of tumor cells histone genes, then damaging tumor cell chromosome and killing tumor cells.
酪丝缬肽（YSV）是一种由酪氨酰-丝氨酰-缬氨酸组成的生物活性三肽。在本研究中，我们研究了YSV对移植于BALB/c（nu/nu）裸鼠体内的人肝癌BEL-7402肿瘤的影响，并通过基因芯片分析肿瘤细胞中的基因表达。结果显示，与对照组相比，YSV显著抑制裸鼠（n = 12）体内移植的人肝癌BEL-7402的生长（P < 0.05）；在320微克/千克/天时抑制率为55%。YSV组和对照组之间有781个基因存在差异。52个基因的表达水平变化了一倍或更多，其中包括37个下调基因和15个上调基因。可能是YSV通过抑制肿瘤细胞组蛋白基因的表达，进而破坏肿瘤细胞染色体并杀死肿瘤细胞，从而表现出显著的抗肿瘤活性。
