用于筛选人源Ras-Raf蛋白相互作用抑制剂的酵母双杂交筛选方法的开发。
Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.
作者信息
Khazak Vladimir, Golemis Erica A, Weber Lutz
机构信息
NexusPharma, Langhorne, PA, USA.
出版信息
Methods Mol Biol. 2005;310:253-71. doi: 10.1007/978-1-59259-948-6_18.
Abstract
A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
摘要
开发了一种酵母双杂交筛选系统，以筛选抑制Ras和Raf蛋白相互作用的小分子。创建了可用于双杂交系统高通量筛选（HTS）的高通透性酵母菌株。通过对Ras-Raf相互作用与hsRPB4-hsRPB7相互作用的差异抑制，得以鉴定出选择性抑制剂。
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