Kimura Takumi, Kawai Kiyohiko, Majima Tetsuro
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Org Lett. 2005 Dec 22;7(26):5829-32. doi: 10.1021/ol052473m.
[graph: see text] We describe the synthesis of new environmentally sensitive fluorescence probes to elucidate DNA structures. DNA oligonucleotides containing fluorophore dan (6-(dimethylamino)-2-acylnaphthalene)-modified dC or dG were able to monitor the microenvironmental changes in both the major and minor grooves of DNA with a B- to A-DNA conformational transition and RNA hybridization.
[图表：见正文] 我们描述了用于阐明DNA结构的新型环境敏感荧光探针的合成。含有荧光团dan（6-（二甲基氨基）-2-酰基萘）修饰的dC或dG的DNA寡核苷酸能够监测DNA从B型到A型构象转变以及RNA杂交过程中主沟和副沟的微环境变化。
