Cuevas P, Díaz-González D, Giménez-Gallego G, Dujovny M
Departamento de Investigación, Hospital Universitario Ramón y Cajal, Universidad de Alcala de Henares, Madrid, Spain.
Neurol Res. 2005 Dec;27(8):797-800. doi: 10.1179/016164105X63665.
Dihydroxy-2,5 benzenesulphonate (dobesilate) is used as an oral agent for treatment of vascular complications of diabetic retinopathy. We previously showed that blockade of fibroblast growth factor (FGF) driving angiogenesis with dobesilate inhibited new blood vessel formation in a mouse gelatine plug assay. In the present study we assessed the effects of dobesilate in rat glioma cells.
Rat C6 cells line were grown as adherent cells in Dulbecco modified Eagle medium supplemented with 1% (v/v) fetal bovine serum and antibiotics. Calcium dobesilate was added in independent experiments at the following concentrations: 10, 25, 50, 75 and 100 microM, and cells were incubated for 24 hours. Effects of dobesilate in glioma cell proliferation and survival were assessed using crystal violet staining and TUNEL assay, respectively.
Incubation of glioma cells with dobesilate for 24 hours concentration-dependently decreased cell proliferation with an apparent IC50 of 25 microM, and this antiproliferative effect was related to a significant increase in glioma cell apoptosis.
The results suggest that dobesilate is a promising candidate leading to the development of a new adjuvant therapeutic strategy for gliomas.
二羟基 - 2,5 - 苯磺酸盐（羟苯磺酸钙）用作治疗糖尿病视网膜病变血管并发症的口服药物。我们之前表明，在小鼠明胶塞试验中，用羟苯磺酸钙阻断驱动血管生成的成纤维细胞生长因子（FGF）可抑制新血管形成。在本研究中，我们评估了羟苯磺酸钙对大鼠胶质瘤细胞的影响。
大鼠C6细胞系在补充有1%（v/v）胎牛血清和抗生素的杜氏改良伊格尔培养基中作为贴壁细胞生长。在独立实验中加入以下浓度的羟苯磺酸钙：10、25、50、75和100微摩尔，细胞孵育24小时。分别使用结晶紫染色和TUNEL检测评估羟苯磺酸钙对胶质瘤细胞增殖和存活的影响。
胶质瘤细胞与羟苯磺酸钙孵育24小时后，细胞增殖呈浓度依赖性降低，表观IC50为25微摩尔，这种抗增殖作用与胶质瘤细胞凋亡显著增加有关。
结果表明，羟苯磺酸钙是一种有前景的候选药物，可用于开发新的胶质瘤辅助治疗策略。
