肥大细胞糜蛋白酶降低屏障功能并抑制角膜上皮细胞的迁移。
Mast cell chymase decreases the barrier function and inhibits the migration of corneal epithelial cells.
作者信息
Ebihara Nobuyuki, Funaki Toshinari, Murakami Akira, Takai Shinji, Miyazaki Mizuo
机构信息
Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
出版信息
Curr Eye Res. 2005 Dec;30(12):1061-9. doi: 10.1080/02713680500346625.
PURPOSE
We investigated the in vitro effects of human mast cell chymase on corneal epithelial cells.
METHODS
Human corneal epithelial cells were incubated with human chymase at activity levels that were likely to exist in the tears of patients with vernal keratoconjunctivitis.
RESULTS
Incubation of chymase resulted in a decrease of barrier function of corneal epithelium. Occludin protein was cleaved by chymase. In the wound assay, incubation with chymase resulted in an inhibition of cell migration.
CONCLUSION
Human chymase causes the proteolysis of occludin and fibronectin, resulting in a decrease of barrier function and inhibition of the migration of corneal epithelial cells.
目的
我们研究了人肥大细胞糜蛋白酶对角膜上皮细胞的体外作用。
方法
将人角膜上皮细胞与人糜蛋白酶一起孵育，其活性水平可能存在于春季角结膜炎患者的泪液中。
结果
糜蛋白酶孵育导致角膜上皮屏障功能下降。封闭蛋白被糜蛋白酶切割。在伤口试验中，与糜蛋白酶孵育导致细胞迁移受到抑制。
结论
人糜蛋白酶导致封闭蛋白和纤连蛋白的蛋白水解，导致屏障功能下降和角膜上皮细胞迁移受到抑制。
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