Thammana P, Gaito A L, Largen M T
Glasgow Research Laboratory, Medical Products E. I. du Pont de Nemours and Company Inc., Newark, DE 19714-6101.
Mol Immunol. 1992 Jul-Aug;29(7-8):1025-8. doi: 10.1016/0161-5890(92)90142-k.
We have determined the nucleotide sequences of the variable regions of H and L chains of a monoclonal antibody 98QQ that interacts with the thyroid hormone triiodo-L- thyronine with high affinity. Analysis of the nucleotide sequence of the light chain V region of 98QQ revealed that the VL sequence is 99% identical to Balb/c germline Vk 21-E sequence. That is an interesting finding with this high affinity anti-T3 antibody, since occurence of predominantly germline variable region sequences is observed in some autoantibodies to self antigens but not usually in high affinity IgG antibodies. The sequence analysis also revealed that the heavy chain variable region sequence of 98QQ is similar to a V region of an anti-DNA antibody (MRL DNA 22). Thus the sequence analysis of our anti-T3 mAb 98QQ has revealed some features of autoantibodies to self antigens.
我们已经确定了单克隆抗体98QQ的重链和轻链可变区的核苷酸序列，该抗体与甲状腺激素三碘-L-甲状腺原氨酸具有高亲和力。对98QQ轻链V区核苷酸序列的分析表明，VL序列与Balb/c种系Vk 21-E序列有99%的同一性。对于这种高亲和力的抗T3抗体来说，这是一个有趣的发现，因为在一些针对自身抗原的自身抗体中观察到主要是种系可变区序列的出现，但在高亲和力的IgG抗体中通常不会出现。序列分析还表明，98QQ的重链可变区序列与一种抗DNA抗体（MRL DNA 22）的V区相似。因此，我们的抗T3单克隆抗体98QQ的序列分析揭示了针对自身抗原的自身抗体的一些特征。
