Ashton John C, Friberg Deborah, Darlington Cynthia L, Smith Paul F
Department of Pharmacology and Toxicology, School of Medical Sciences, University of Otago Medical School, University of Otago, Dunedin, New Zealand.
Neurosci Lett. 2006 Mar 27;396(2):113-6. doi: 10.1016/j.neulet.2005.11.038. Epub 2005 Dec 13.
Reports of cannabinoid CB2 receptor protein in the brain have been ambiguous. We therefore tested for CB2 immunoreactivity in the rat brain using immunofluorescence. We detected CB2 labeling in fine fibers in the granule layer. This CB2 labeling did not co-localise with the astrocyte marker glial fibrillary acidic protein (GFAP) and, therefore, the CB2-positive fibers were not astrocytes and were possibly microglial or neuronal. Additionally, strong CB2 labeling was detected in capillary endothelia in the granule, Purkinje cell, and molecular layers. Our results suggest that the role of CB2 receptors in the brain may have been previously underestimated.
关于大脑中大麻素CB2受体蛋白的报道一直含糊不清。因此，我们使用免疫荧光法检测了大鼠大脑中的CB2免疫反应性。我们在颗粒层的细纤维中检测到了CB2标记。这种CB2标记与星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）不共定位，因此，CB2阳性纤维不是星形胶质细胞，可能是小胶质细胞或神经元。此外，在颗粒层、浦肯野细胞层和分子层的毛细血管内皮中检测到了强烈的CB2标记。我们的结果表明，CB2受体在大脑中的作用可能此前被低估了。
