Alves S P, Marcelino C, Portugal P V, Bessa R J B
Estação Zootécnica Nacional, Instituto Nacional de Investigação Agrária e das Pescas, 2005-048 Vale de Santarém, Portugal.
J Dairy Sci. 2006 Jan;89(1):170-3. doi: 10.3168/jds.S0022-0302(06)72081-1.
Heptadecenoic acid (17:1) is a minor constituent of ruminant fats and its isomeric definition remains undefined in most reports on ruminant milk and intramuscular fat. Samples of milk and intramuscular fat of bovine, ovine, and caprine origin were analyzed by gas chromatography (GC) using 3 capillary columns with and without addition of 17:1 cis-10. Additionally, cis isomers of ovine milk fat samples were isolated as methyl esters by preparative thin-layer chromatography and analyzed by GC. The structural analysis of 17:1 present in samples was achieved by chemical ionization tandem mass spectrometry techniques. The isomer 17:1 cis-9 is the overwhelming heptadecenoic isomer in ruminant milk and intramuscular fat; 17:1 cis-10 is virtually absent. Moreover, current GC methods were able to resolve cis-9 from cis-10 and cis-8 isomers, so reports on 17:1 contents in ruminant fat should define its isomeric composition.
十七碳烯酸（17:1）是反刍动物脂肪中的一种次要成分，在大多数关于反刍动物乳汁和肌内脂肪的报告中，其异构体定义仍不明确。使用3根毛细管柱，在添加和不添加顺式-10 17:1的情况下，通过气相色谱法（GC）对牛、羊和山羊来源的乳汁和肌内脂肪样本进行了分析。此外，通过制备型薄层色谱法将羊乳脂肪样本的顺式异构体分离为甲酯，并通过GC进行分析。通过化学电离串联质谱技术对样本中存在的17:1进行了结构分析。异构体17:1顺式-9是反刍动物乳汁和肌内脂肪中占主导地位的十七碳烯酸异构体；几乎不存在17:1顺式-10。此外，当前的GC方法能够将顺式-9与顺式-10和顺式-8异构体区分开来，因此关于反刍动物脂肪中17:1含量的报告应明确其异构体组成。
