Cöplü Nilay, Esen Berrin, Kurtoğlu Demet, Gözalan Aysegül, Miyamura Kikuko, Yoshida Iwao
Refik Saydam Hifzissihha Merkezi Başkanliği, Salgin Hastaliklar Araştirma Müdürlüğü, Ankara.
Mikrobiyol Bul. 2005 Jul;39(3):281-9.
Seroepidemiological studies need sensitive, practicle and cost-effective methods. For pertussis serosurveillance, an in-house ELISA for antipertussis-toxin (PT) and anti-filamentous-hemagglutinin (FHA) were established in our laboratory and compared by a Ball-ELISA which had been reported to be reliable previously. Sixty sera with various antibody titers were tested by both of the methods. The correlation coefficients between two methods were 0.729 and 0.776 for anti-PT and anti-FHA, respectively, and regression coefficients were 0.623 and 0.693, respectively. The in-house ELISA was applied to a serosurvey including 373 healthy subjects (6 months-91 years old) in Turkey to observe the results. The moving averages of both antibodies were increased until 10 years old, reaching to 31 EU/ml for anti-PT and 65 EU/ml for anti-FHA and kept around this level in the older ages. The in-house ELISA was found to be reliable and the serosurvey results obtained by ELISA showed a characteristic distribution of antibody titers in each age group.
血清流行病学研究需要灵敏、实用且经济高效的方法。对于百日咳血清监测，我们实验室建立了一种用于检测抗百日咳毒素（PT）和抗丝状血凝素（FHA）的内部酶联免疫吸附测定（ELISA），并与之前报道可靠的球ELISA进行比较。用这两种方法检测了60份具有不同抗体效价的血清。抗PT和抗FHA两种方法之间的相关系数分别为0.729和0.776，回归系数分别为0.623和0.693。将内部ELISA应用于对土耳其373名健康受试者（6个月至91岁）的血清调查以观察结果。两种抗体的移动平均值在10岁之前均升高，抗PT达到31 EU/ml，抗FHA达到65 EU/ml，在较大年龄组中保持在该水平左右。发现内部ELISA可靠，通过ELISA获得的血清调查结果显示了每个年龄组抗体效价的特征性分布。
