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染料亲和配体修饰的醋酸纤维滤棒对白蛋白的等温吸附研究

Study of isothermal adsorption of albumin on acetate fiber filter rod with dye affinity ligand.

作者信息

Li Jing-Hua, Wang Jun-De, Liu Xue-Liang

机构信息

Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.

出版信息

Se Pu. 2002 Sep;20(5):419-22.

Abstract

Cibacron Blue F3GA was immobilized onto an acetate fiber filter rod to produce potential affinity matrix. The adsorption isotherms of the prepared matrix for either bovine serum albumin or human serum albumin were investigated. It was experimentally shown that the affinity isothermal adsorption obeyed Freundlich adsorption model. By means of affinity chromatography of human plasma using the prepared matrix, purified human serum albumin was obtained.

摘要

将汽巴克隆蓝F3GA固定在醋酸纤维滤棒上,制备潜在的亲和基质。研究了所制备基质对牛血清白蛋白或人血清白蛋白的吸附等温线。实验表明,亲和等温吸附符合弗罗因德利希吸附模型。利用所制备的基质对人血浆进行亲和层析,得到了纯化的人血清白蛋白。

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