Lu Qi-Lin
Department of Chemistry and Environmental Science, Jinzhou Teachers College, Jinzhou 121000, China.
Se Pu. 2002 Sep;20(5):464-6.
An improved method for the determination of benzoyl peroxide in wheat flour is reported. Benzoyl peroxide was extracted with petroleum ether, and then analysed by reversed-phase high performance liquid chromatography on a Shim-Pack VP-ODS column (0.46 cm i.d. x 15 cm, 5 microm - 6 microm) using a mobile phase of acetonitrile-0.3% H3PO4 aqueous solution (4:1 in volume ratio) with a flow rate of 1.0 mL/min, and UV-236 nm detection. A linear correlation was observed between 0.002 g/L- 0.012 g/L of benzoyl peroxide. The detection limit was 0.005 g/kg. This proposed method without redox reaction is superior to colorimetric determination. This method is characterized with simplicity, convenience, reproducibility and accuracy.
报道了一种测定小麦粉中过氧化苯甲酰的改进方法。过氧化苯甲酰用石油醚萃取，然后在Shim-Pack VP-ODS柱（内径0.46 cm×15 cm，5μm - 6μm）上通过反相高效液相色谱法进行分析，流动相为乙腈-0.3%磷酸水溶液（体积比4:1），流速为1.0 mL/min，采用236 nm紫外检测。在0.002 g/L - 0.012 g/L的过氧化苯甲酰浓度之间观察到线性相关性。检测限为0.005 g/kg。该方法无需氧化还原反应，优于比色法测定。该方法具有简单、方便、重现性好和准确性高的特点。
