Iakovleva O V, Talipova E V, Kukarskikh G P, Krendeleeva T E, Rubin A B
Biofizika. 2005 Nov-Dec;50(6):1112-9.
The functional state of medicinal plants of Convallaria majalis L., Vaccinium vitis-idaeae L., Arctostaphylos uva-ursi L. in connection with heavy metal accumulation in their leaves under man impact was studied by the pulse-amplitude-modulation (PAM) fluorometric method. The relative yield of variable fluorescence (F(v)/F(m)), induction of fluorescence of chlorophyll, and fluorescence quenching processes in leaves at different distances from the local Kirov-Sovetsk, Kirov-Omutninsk road in Kirov region were analyzed. Changes in biophysical characteristics with the increasing content of heavy metals in leaves were demonstrated. The most informative characteristic is F(v)/F(m). Its value correlates with the activity of the photosynthetic apparatus and reflects the potential effeciency of photosynthesis. The better are the environmental conditions of plant growth, the higher is the F(v)/F(m) ratio and the lower is its average statistical deviation. Fluorescence induction curves do not always vary in shape under our ecological conditions, indicating relatively favorable conditions at places of plant growth investigated. The rate of the environmental pollution in the investigated region is not critical, since the content of heavy metal in leaves does not change considerably with the distance from the road.
采用脉冲幅度调制（PAM）荧光法研究了铃兰、越橘、熊果叶在人为影响下其叶片重金属积累与药用植物功能状态之间的关系。分析了基洛夫地区基洛夫 - 苏维埃茨克、基洛夫 - 奥穆特宁斯克公路沿线不同距离处叶片中可变荧光相对产量（F(v)/F(m)）、叶绿素荧光诱导以及荧光猝灭过程。结果表明，随着叶片中重金属含量的增加，生物物理特性发生了变化。最具信息量的特征是F(v)/F(m)。其值与光合机构的活性相关，反映了光合作用的潜在效率。植物生长的环境条件越好，F(v)/F(m)比值越高，其平均统计偏差越低。在我们的生态条件下，荧光诱导曲线的形状并非总是变化，这表明所研究的植物生长地的条件相对有利。由于叶片中重金属含量随离道路距离的变化不大，所以研究区域的环境污染程度并不严重。
