Manyś-Kubacka K, Kostrzewa A, Sobieska M, Samborski W
Department of Ophthalmology, University of Medical Sciences, Poznań, Poland.
Rocz Akad Med Bialymst. 2005;50:289-92.
The alterations of lymphocyte subpopulations assessment after surgery in choroidal melanoma patients compared to cataract patients.
12 patients with malignant melanoma of the choroid, 10 patients subjected to surgery due to cataract. Methods--flow cytometric measurement of absolute lymphocyte count, the number of all T cells (CD3+), T helper lymphocytes (CD3+ CD4+), T cytotoxic lymphocytes (CD3+CD8+), B lymphocytes (CD19+), NK cells (CD3-CD16+) and T cells (CD3+) cells with gammasigma TCR, on the day of surgery and two days after it.
Comparable numbers of cells were observed in both groups prior to surgery, but the behavior of some populations differed: CD3+, CD3+CD4+ cells increased in melanoma patients whereas they decreased in reference group, the number of T lymphocytes with gammasigma TCR was significantly higher in melanoma patients before surgery and it did not differ after it.
Though there were no significant differences in lymphocyte subpopulations between melanoma patients and the reference group, it seems that the presence of tumour influences the reactivity of the immune system to the trauma (surgery).
比较脉络膜黑色素瘤患者与白内障患者术后淋巴细胞亚群评估的变化。
12例脉络膜恶性黑色素瘤患者，10例因白内障接受手术的患者。方法——在手术当天及术后两天，通过流式细胞术测量淋巴细胞绝对计数、所有T细胞（CD3 +）、辅助性T淋巴细胞（CD3 + CD4 +）、细胞毒性T淋巴细胞（CD3 + CD8 +）、B淋巴细胞（CD19 +）、NK细胞（CD3 - CD16 +）以及带有γδTCR的T细胞（CD3 +）的数量。
两组术前观察到的细胞数量相当，但部分细胞群的变化不同：黑色素瘤患者中CD3 +、CD3 + CD4 +细胞增加，而对照组中则减少；黑色素瘤患者术前带有γδTCR的T淋巴细胞数量显著更高，术后无差异。
虽然黑色素瘤患者与对照组之间淋巴细胞亚群无显著差异，但肿瘤的存在似乎影响了免疫系统对创伤（手术）的反应性。
