Das Arpita, Chakraborti Phuljhuri, Chatterjee Urmimala, Monddal Gautam, Chatterjee Bishnu P
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India.
Indian J Exp Biol. 2005 Dec;43(12):1170-5.
Enzymed-linked immunosorbent assay of hilsa and pomfret muscle extracts showed specific IgE binding to ten allergic patients' sera, the results corroborated to that of skin prick test. Comparison of allergen profiles of the two fish extracts by immunoblotting revealed a common antigenic protein of 50 kDa and some high molecular weight fish allergens instead of low molecular weight parvalbumin found in several fishes. Purified and well characterized fish allergens are always considered better than crude fish extracts for diagnostic use.
对鲥鱼和鲳鱼肌肉提取物进行的酶联免疫吸附测定显示，十种过敏患者血清与特异性IgE结合，结果与皮肤点刺试验相符。通过免疫印迹比较两种鱼类提取物的过敏原谱，发现了一种50 kDa的共同抗原蛋白和一些高分子量鱼类过敏原，而不是几种鱼类中发现的低分子量小清蛋白。纯化且特征明确的鱼类过敏原在诊断用途上总是被认为比粗制鱼类提取物更好。
