Reinhard Hans, Rupp Heinz, Sager Fritz, Streule Michael, Zoller Otmar
Swiss Federal Office of Public Health, Division of Food Science, CH-3003 Bern, Switzerland.
J Chromatogr A. 2006 Apr 21;1112(1-2):353-60. doi: 10.1016/j.chroma.2005.11.079. Epub 2005 Dec 15.
In recent years there has been growing interest in replacing (genetically modified) soya by lupin. Lupin seeds, flours and lupin containing food have been analyzed in order to assess the relevance of a potential health hazard given by mycotoxins and/or naturally occurring alkaloids. Since not all important alkaloids used for quantitation were commercially available, isolation of lupanine, 13alpha-hydroxylupanine and angustifoline from lupin flours of high alkaloid contents was performed. Alkaloids were analyzed by GC-MS/GC-FID in parallel, while the phomopsin mycotoxins were analyzed by ELISA, since chromatographic methods were not sensitive enough and required time-consuming sample cleanup. The analyzed lupin containing foods were free of phomopsins. In foods where lupin was only a minor constituent the alkaloid content was of no concern. However, roasted lupin beans intended as coffee surrogate had alkaloid contents close to the Australian intervention limit of 200 microg/g.
近年来，用羽扇豆替代（转基因）大豆的兴趣日益浓厚。为了评估霉菌毒素和/或天然生物碱可能带来的健康危害的相关性，对羽扇豆种子、面粉及含羽扇豆的食品进行了分析。由于并非所有用于定量的重要生物碱都有商业供应，因此从高生物碱含量的羽扇豆面粉中分离出了羽扇豆碱、13α-羟基羽扇豆碱和狭叶羽扇豆碱。生物碱通过气相色谱-质谱联用/气相色谱-火焰离子化检测器平行分析，而鬼笔环肽类霉菌毒素则通过酶联免疫吸附测定法分析，因为色谱方法不够灵敏，且需要耗时的样品净化。分析的含羽扇豆食品不含鬼笔环肽。在羽扇豆仅为次要成分的食品中，生物碱含量无需担忧。然而，用作咖啡替代品的烘焙羽扇豆生物碱含量接近澳大利亚200微克/克的干预限值。
