de Chaldée M, Brochier C, Van de Vel A, Caudy N, Luthi-Carter R, Gaillard M C, Elalouf J M
Laboratoire de Physiogénomique, Service de Biochimie et de Génétique Moléculaire, Commissariat à l'Energie Atomique, Saclay, 91191 Gif-sur-Yvette Cedex, France.
Genomics. 2006 Feb;87(2):200-7. doi: 10.1016/j.ygeno.2005.10.009. Epub 2005 Dec 15.
In an initial study, we compared quantitative transcriptome data across mouse brain territories using the serial analysis of gene expression method. Among the novel regional markers that we discovered, we focused on a striatum-enriched transcript with no available experimental cDNA sequence. Here, we report its cloning, gene structure, and detailed distribution in mouse brain. Quantitative RT-PCR and in situ hybridization demonstrated predominant expression in dorsolateral striatum. We therefore named it capucin for caudate-and putamen-enriched sequence. Mouse capucin is a 237-amino-acid protein, without any registered ortholog in mammalian species. It contains no recognizable motif other than two predicted carboxy-terminal transmembrane domains. When expressed in fusion with a fluorescent protein, it localized to the Golgi apparatus in two mammalian cell lines. Interestingly, we observed a significant down-regulation of capucin mRNA levels in two rodent models of Huntington disease, indicating a possible contribution to the pathogenesis of this disorder.
在一项初步研究中，我们使用基因表达序列分析方法比较了小鼠大脑各区域的定量转录组数据。在我们发现的新型区域标记物中，我们重点关注了一种纹状体富集转录本，其尚无可用的实验性cDNA序列。在此，我们报告其克隆、基因结构以及在小鼠大脑中的详细分布。定量逆转录聚合酶链反应（RT-PCR）和原位杂交显示其在背外侧纹状体中主要表达。因此，我们将其命名为capucin，即尾状核和壳核富集序列。小鼠capucin是一种由237个氨基酸组成的蛋白质，在哺乳动物物种中没有任何已注册的直系同源物。除了两个预测的羧基末端跨膜结构域外，它不包含任何可识别的基序。当与荧光蛋白融合表达时，它定位于两种哺乳动物细胞系的高尔基体。有趣的是，我们在两种亨廷顿病啮齿动物模型中观察到capucin mRNA水平显著下调，表明其可能参与了该疾病的发病机制。
