Britt B A, Endrenyi L, Cadman D L, Fan H M, Fung H Y
Anesthesiology. 1975 Mar;42(3):292-300.
Vastus lateralis muscle was excised from normal pigs and from pigs susceptible to malignant hyperthermia. Anesthesia consisted of diazepam, N2O:O2, and a lidocaine field block. In the susceptible (MHS) pigs, respiratory control in mitochondria of excised muscle was normal, while electron transport was accelerated. Glutamate log Q3's and log Q4's were in inverse linear relationship to porcine weights. In the presence of glutamate, state 3 respiration was 3.5 times, and state 4 respiration 3.2 times, higher in MHS than in normal mitochondria (independent of weight or halothane dose). Thus, the MHS mitochondria were able to produce ATP more efficiently than normal mitochondria. In vitro, halothane inhibited glutamate Q3's and R.C.I.'s, slightly increased succinate Q3's and R.C.I.'s, had no significant effect on glutamate or succinate Q4's, and, moderately lowered glutamate and succinate P/O ratios. These changes were similar to those observed in normal pigs. Calcium uptake into MHS mitochondria was markedly less than normal but was not significantly altered by in-vitro halothane. These results suggest a mitochondrial membrane component for the defect of procine malignant hyperthermia since calcium is normally associated with the formation of the phospholipid lattice of this membrane. (Key words: Hyperthermia, malignant; Metabolism, mitochondrial; Muscle, skeletal, mitochondria; Anesthetics, volatile, halotbane; Ions, calcium.)
从正常猪和易患恶性高热的猪身上切除股外侧肌。麻醉采用地西泮、N₂O:O₂和利多卡因局部阻滞。在易患恶性高热（MHS）的猪中，切除肌肉线粒体的呼吸控制正常，而电子传递加速。谷氨酸的log Q₃和log Q₄与猪的体重呈反线性关系。在谷氨酸存在的情况下，MHS猪的状态3呼吸比正常线粒体高3.5倍，状态4呼吸高3.2倍（与体重或氟烷剂量无关）。因此，MHS线粒体比正常线粒体更能有效地产生ATP。在体外，氟烷抑制谷氨酸的Q₃和呼吸控制率（R.C.I.），略微增加琥珀酸的Q₃和R.C.I.，对谷氨酸或琥珀酸的Q₄无显著影响，并适度降低谷氨酸和琥珀酸的磷氧比。这些变化与在正常猪中观察到的相似。MHS线粒体对钙的摄取明显低于正常，但体外氟烷对其无显著改变。这些结果表明，猪恶性高热缺陷存在线粒体膜成分，因为钙通常与该膜的磷脂晶格形成有关。（关键词：高热，恶性；代谢，线粒体；肌肉，骨骼，线粒体；麻醉剂，挥发性，氟烷；离子，钙。）
